Breast organoid suspension cultures maintain long-term estrogen receptor expression and responsiveness.

ABSTRACT: Organoid cultures offer a powerful technology to investigate many different aspects of development, physiology, and pathology of diverse tissues. Unlike standard tissue culture of primary breast epithelial cells, breast organoids preserve the epithelial lineages and architecture of the normal tissue. However, existing organoid culture methods are tedious, difficult to scale, and do not robustly retain estrogen receptor (ER) expression and responsiveness in long-term culture. Here, we describe a modified culture method to generate and maintain organoids as suspension cultures in reconstituted basement membrane (™Matrigel). This method improves organoid growth and uniformity compared to the conventional Matrigel dome embedding method, while maintaining the fidelity of the three major epithel

SUBMITTER: Chang KC 

PROVIDER: S-EPMC11659324 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature