A family of NADPH/NADP⁺ biosensors reveals in vivo dynamics of central redox metabolism across eukaryotes.

ABSTRACT: The NADPH/NADP⁺ redox couple is central to metabolism and redox signalling. NADP redox state is differentially regulated by distinct enzymatic machineries at the subcellular compartment level. Nonetheless, a detailed understanding of subcellular NADP redox dynamics is limited by the availability of appropriate tools. Here, we introduce NAPstars, a family of genetically encoded, fluorescent protein-based NADP redox state biosensors. NAPstars offer real-time, specific measurements, across a broad-range of NADP redox states, with subcellular resolution. NAPstar measurements in yeast, plants, and mammalian cell models, reveal a conserved robustness of cytosolic NADP redox homoeostasis. NAPstars uncover cell cycle-linked NADP redox oscillations in yeast and illumination- and hypoxia-

SUBMITTER: Scherschel M 

PROVIDER: S-EPMC11659435 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature