Modeling of cell cultivation for monoclonal antibody production processes considering lactate metabolic shifts.

ABSTRACT: Demand for monoclonal antibodies (mAbs) is rapidly increasing. To achieve higher productivity, there have been improvements to cell lines, operating modes, media, and cultivation conditions. Representative mathematical models are needed to narrow down the growing number of process alternatives. Previous studies have proposed mechanistic models to depict cell metabolic shifts (e.g., lactate production to consumption). However, the impacts of variations of some operating conditions have not yet been fully incorporated in such models. This paper offers a new mechanistic model considering variations in dissolved oxygen and glutamine depletion on cell metabolism applied to a novel Chinese hamster ovary (CHO) cell line. Expressions for the specific rates of lactate production, glutamine consumpt

SUBMITTER: Okamura K 

PROVIDER: S-EPMC11659809 | biostudies-literature | 2024 Nov-Dec

REPOSITORIES: biostudies-literature