De novo sequencing allows genome-wide identification of genes involved in galactomannan synthesis in locust bean (Ceratonia siliqua).

ABSTRACT: Locust bean (Ceratonia siliqua) accumulates the galactomannan (GM) locust bean gum (LBG) in its seeds. LBG is a major industrial raw material used as a food thickener and gelling agent, whose unique properties mean that it cannot be readily replaced by other GMs. Whereas much is known about GM accumulation and the genes associated with GM biosynthesis in legumes, the genes involved in GM biosynthesis in C. siliqua are largely unknown. Here, we present a genome-wide list of genes predicted to be associated with the GM biosynthesis pathway in C. siliqua. We confirmed high GM accumulation in endosperm using a newly established GM quantification method involving LC-MS/MS. Through de novo draft genome assembly, we comprehensively identified genes predicted to be related to the GM biosynthesis p

SUBMITTER: Akutsu M 

PROVIDER: S-EPMC11659883 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature