Proteomic analysis for busulfan-induced spermatogenesis disorder.

ABSTRACT:

Background

Busulfan is the most commonly used drug for the treatment of chronic myelogenous leukemia and pretreatment for hematopoietic stem cell transplantation, which can damage the reproductive and immune system. However, little is known about the protein expression profiling in busulfan treated testis.

Methods

This research studies the proteomics for busulfan-induced spermatogenesis disorder. The model of busulfan-induced mouse spermatogenesis disorder was subjected to label-free quantification proteomics analysis. Clustering heatmap, gene ontology, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and protein interaction analyses were performed and validated by molecular experiments.

Results

The busulfan-treated mouse model showed abnormal testis morphology

SUBMITTER: Hu K 

PROVIDER: S-EPMC11660421 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature