Developmental maturation and regional heterogeneity but no sexual dimorphism of the murine CNS myelin proteome.

ABSTRACT: The molecules that constitute myelin are critical for the integrity of axon/myelin-units and thus speed and precision of impulse propagation. In the CNS, the protein composition of oligodendrocyte-derived myelin has evolutionarily diverged and differs from that in the PNS. Here, we hypothesized that the CNS myelin proteome also displays variations within the same species. We thus used quantitative mass spectrometry to compare myelin purified from mouse brains at three developmental timepoints, from brains of male and female mice, and from four CNS regions. We find that most structural myelin proteins are of approximately similar abundance across all tested conditions. However, the abundance of multiple other proteins differs markedly over time, implying that the myelin proteome matures bet

SUBMITTER: Siems SB 

PROVIDER: S-EPMC11660532 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature