Mir-615-3p promotes osteosarcoma progression via the SESN2/AMPK/mTOR pathway.

ABSTRACT:

Background

Osteosarcoma (OS) is the most common primary malignant bone neoplasm. Growing researches have highlighted the tumor promoting role of miR-615-3p in various cancers. Notwithstanding, the biological function and underlying mechanisms of miR-615-3p in OS development still unclear.

Methods

Quantitative Real-Time PCR analysis (qRT-PCR) and RNA fluorescence in situ hybridization (FISH) staining were performed to measure miR-615-3p expression in OS. CCK-8 assay, colony formation assay and EdU assay were applied to analyze the OS cell proliferation activity. Cell metastasis abilities were evaluated using Transwell assays. Analysis of apoptosis was performed based on flow cytometric detection. The potential mechanisms of miR-615-3p in OS progression were investigated throu

SUBMITTER: Yu X 

PROVIDER: S-EPMC11660548 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature