Isolation method of brain microvessels from small frozen human brain tissue for blood-brain barrier protein expression analysis.

ABSTRACT:

Background

Protein expression analysis of isolated brain microvessels provides valuable insights into the function of the blood-brain barrier (BBB). However, isolation of brain microvessels from human brain tissue, particularly in small quantities, poses significant challenges. This study presents a method for isolating brain microvessels from a small amount of frozen human brain tissue, adapting techniques from an established mouse brain capillary isolation method.

Methods

Brain microvessel fractions were obtained from approximately 0.3 g of frozen human brain tissue (frontal cortex) using a bead homogenizer for homogenization, followed by purification with a combination of cell strainers and glass beads. Protein expression in the isolated human microvessel fractions and wh

SUBMITTER: Ogata S 

PROVIDER: S-EPMC11660552 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature