Effects of different temperatures on chondrocyte growth: a transcriptomic analysis.

ABSTRACT:

Background

Our previous study demonstrated that temperature-related microwave ablation (MWA) can safely modulate growth plates of piglets' vertebrae. Therefore, this study is designed to investigate the effects of different temperatures on chondrocyte viability and the underlying molecular mechanisms in vitro.

Methods

Following a 10-minute treatment at different temperatures (37 °C, 40 °C, 42 °C, 44 °C, 46 °C, 48 °C, and 50 °C), CCK-8 assay was used to examine the viability of ATDC5 cells at 12 h. Differentially expressed genes (DEGs) and the hub genes in ATDC5 cells treated at 37 °C, 40 °C and 44 °C were identified using RNA-seq. The expression of hub genes in ATDC5 cells was validated using RT-qPCR.

Results

Compared with 37 °C, exposure to 40 °C significantly increa

SUBMITTER: Zhao W 

PROVIDER: S-EPMC11660629 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature