Compression force promotes the osteogenic differentiation of periodontal ligament stem cells by regulating NAT10-mediated ac4C modification of BMP2.

ABSTRACT:

Background

Orthodontic treatment applies specific corrective forces to teeth, transmitting stress to periodontal tissue, thereby regulating the growth and development of periodontal ligament stem cells (PDLSCs). Recently, N-acetyltransferase 10 (NAT10) mediated N4-acetylcytidine (ac4C) modification is demonstrated to play a key role in the osteogenic differentiation of stem cells. Therefore, this study aimed explore the effects of Orthodontic treatment on the NAT10 mediated ac4C modification and osteogenic differentiation of PDLSCs.

Methods

Compressive force was used to treat PDLSCs to simulate orthodontic force treatment. The ALP and ARS staining was performed to analyze the osteogenic differentiation of PDLSCs. Besides, ac4C dot blot and ac4C-RIP assays were performed to d

SUBMITTER: Feng Y 

PROVIDER: S-EPMC11660852 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature