Linking the gut microbiome to host DNA methylation by a discovery and replication epigenome-wide association study.

ABSTRACT: Microbiome influences multiple human systems, but its effects on gene methylation is unknown. We investigated the relations between gene methylation in blood and the abundance of common gut bacteria profiled by 16s rRNA gene sequencing in two population-based Dutch cohorts: LifeLines-Deep (LLD, n = 616, discovery) and the Netherlands Twin Register (NTR, n = 296, replication). In LLD, we also explored microbial pathways using data generated by shotgun metagenomic sequencing (n = 683). Methylation in both cohorts was profiled in blood samples using the Illumina 450K array. Discovery and replication analysis identified two independent CpGs associated with the genus Eggerthella: cg16586104 (P_{meta-analysis} = 3.21 × 10^-11) and cg12234533 (P_{meta-analysis} = 4.29 ×

SUBMITTER: Demirkan A 

PROVIDER: S-EPMC11660878 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature