Multi-omic integration with human DRG proteomics highlights TNFα signalling as a relevant sexually dimorphic pathway.

ABSTRACT: The peripheral nervous system (PNS) plays a critical role in pathological conditions, including chronic pain disorders, that manifest differently in men and women. To investigate this sexual dimorphism at the molecular level, we integrated quantitative proteomic profiling of human dorsal root ganglia (hDRG) and peripheral nerve tissue into the expanding omics framework of the PNS. Using data-independent acquisition (DIA) mass spectrometry, we characterized a comprehensive proteomic profile, validating tissue-specific differences between the hDRG and peripheral nerve. Through multi-omic analyses and in vitro functional assays, we identified sex-specific molecular differences, with TNFα signalling emerging as a key sexually dimorphic pathway with higher prominence in males. Genetic ev

SUBMITTER: Barry AM 

PROVIDER: S-EPMC11661068 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature