Tet2 and tet3 regulate cell fate specification and differentiation events during retinal development.

ABSTRACT: Tet enzymes are epigenetic modifiers that impact gene expression via 5mC to 5hmC oxidation. Previous work demonstrated the requirement for Tet and 5hmC during zebrafish retinogenesis. tet2 ^-/- ;tet3 ^-/- mutants possessed defects in the formation of differentiated retinal neurons, but the mechanisms underlying these defects are unknown. Here, we leveraged scRNAseq technologies to better understand cell type-specific deficits and molecular signatures underlying the tet2 ^-/- ;tet3 ^-/- retinal phenotype. Our results identified defects in the tet2 ^-/- ;tet3 ^-/- retinae that included delayed specification of several retinal cell types, reduced maturity across late-stage cones, expansions o

SUBMITTER: Heilman SA 

PROVIDER: S-EPMC11661121 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature