Vitrification and rapid rewarming of precision-cut liver slices for pharmacological and biomedical research.

ABSTRACT:

Background and aims

High-throughput in vitro pharmacological toxicity testing is essential for drug discovery. Precision-cut liver slices (PCLS) provide a robust system for screening that is more representative of the complex 3D structure of the whole liver than isolated hepatocytes. However, PCLS are not available as off-the-shelf products, significantly limiting their translational potential. Cryopreservation could solve this bottleneck by effectively preserving PCLS indefinitely until their time of use. Conventional cryopreservation (slow cooling in DMSO-forming ice) results in poor PCLS viability and function and, therefore, has proven unsuitable. Here, we explore an "ice-free" cryopreservation approach called vitrification and focus on culturing and assessing PCLS for 3 days p

SUBMITTER: Ramesh S 

PROVIDER: S-EPMC11661125 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature