Bicoid-nucleosome competition sets a concentration threshold for transcription constrained by genome replication.

ABSTRACT: Transcription factors (TFs) regulate gene expression despite constraints from chromatin structure and the cell cycle. Here we examine the concentration-dependent regulation of hunchback by the Bicoid morphogen through a combination of quantitative imaging, mathematical modeling and epigenomics in Drosophila embryos. By live imaging of MS2 reporters, we find that, following mitosis, the timing of transcriptional activation driven by the hunchback P2 (hb P2) enhancer directly reflects Bicoid concentration. We build a stochastic model that can explain in vivo onset time distributions by accounting for both the competition between Bicoid and nucleosomes at hb P2 and a negative influence of DNA replication on transcriptional elongation. Experimental mod

SUBMITTER: Degen EA 

PROVIDER: S-EPMC11661180 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature