Buffering of nuclear membrane tension and mechanotransduction by the endoplasmic reticulum revealed by quantitative ALPIN imaging.

ABSTRACT: Nuclear deformation by osmotic shock or necrosis activates the cytosolic phospholipase A2 (cPla₂) nuclear shape sensing pathway, a key regulator of tissue inflammation and repair. Ca²⁺ and inner nuclear membrane (INM) tension (T_INM) are believed to mediate nucleoplasmic cPla₂ activation. The concept implies that T_INM persists long enough to stimulate cPla₂-INM adsorption. However, T_INM may instead be rapidly dissipated by the contiguous endoplasmic reticulum (ER), with cPla₂-INM adsorption reporting rather on changes in Ca²⁺ than T_INM. The impact of T_INM and ER contiguity on nuclear shape sensing and mechanotransduction remains unknown. To address this gap, we developed

SUBMITTER: Shen Z 

PROVIDER: S-EPMC11661296 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature