A specific gene expression program underlies antigen archiving by lymphatic endothelial cells in mammalian lymph nodes.

ABSTRACT: Lymph node (LN) lymphatic endothelial cells (LEC) actively acquire and archive foreign antigens. Here, we address questions of how LECs achieve durable antigen archiving and whether LECs with high levels of antigen express unique transcriptional programs. We used single cell sequencing in dissociated LN tissue and spatial transcriptomics to quantify antigen levels in LEC subsets and dendritic cell populations at multiple time points after immunization and determined that ceiling and floor LECs archive antigen for the longest duration. We identify, using spatial transcriptomics, antigen positive LEC-dendritic cell interactions. Using a prime-boost strategy we find increased antigen levels within LECs after a second immunization demonstrating that LEC antigen acquisition and archiving capaci

SUBMITTER: Tamburini B 

PROVIDER: S-EPMC11661310 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature