Deep two-photon voltage imaging with adaptive excitation.

ABSTRACT: Optical imaging of neuronal voltage dynamics is invaluable to studying brain functions. However, high-speed imaging at significant depth is challenging due to the limitations of the short pixel dwell time and the maximum permissible excitation power in tissues. We report high-speed, deep voltage imaging by applying adaptive excitation, which illuminates the regions of interest only. We imaged neuronal voltage activities across two planes down to 500-630 μm depth in the awake mouse brain. Our techniques can be straightforwardly applied to typical two-photon microscopes.

SUBMITTER: Zhao S 

PROVIDER: S-EPMC11661313 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature