Tin prefiltration in computed tomography does not significantly alter radiation-induced gene expression and DNA double-strand break formation.

ABSTRACT:

Background

The tin (Sn) prefilter technique is a recently introduced dose-saving technique in computed tomography (CT). This study investigates whether there is an altered molecular biological response in blood cells using the tin prefiltering technique.

Methods

Blood from 6 donors was X-irradiated ex-vivo with 20 mGy full dose (FD) protocols (Sn 150 kV, 150 kV, and 120 kV) and a tin prefiltered 16.5 mGy low dose (LD) protocol on a CT scanner. Biological changes were determined by quantification of γH2AX DNA double-strand break (DSB) foci, and differential gene expression (DGE) relative to unexposed samples were examined for seven known radiation-induced genes (FDXR, DDB2, BAX, CDKN1A, AEN, EDA2R, APOBEC3H) and 667 microRNAs (miRNA).

Results

EDA2R and DDB2 gene expres

SUBMITTER: Schule S 

PROVIDER: S-EPMC11661594 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature