Lung immune incompetency after mild peritoneal sepsis and its partial restoration by type 1 interferon: a mouse model study.

ABSTRACT:

Background

Sepsis is commonly associated with acute respiratory distress syndrome (ARDS). Although the exaggerated inflammation may damage intact lung tissues, a percentage of patients with ARDS are reportedly immunocompromised, with worse outcomes. Herein, using a murine sepsis model, time-course immune reprogramming after sepsis was evaluated to explore whether the host is immunocompromised. Leukocyte kinetics in the lung tissue were evaluated in a male C57/BL6 mouse model of mild peritoneal sepsis induced by cecal ligation and puncture, with the survival rate exceeds 90%. Lung immune reactivity was evaluated by intratracheal instillation of lipopolysaccharide (LPS; 30 µg). Furthermore, the effect of interferon (IFN)-β in vivo and ex vivo was evaluated.

Results

Four days a

SUBMITTER: Meng Q 

PROVIDER: S-EPMC11662124 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature