Immunomonitoring in bladder cancer: a short report on stability of leukocytes and proteins in refrigerated urine samples.

ABSTRACT:

Background

Urine biomarkers are crucial for monitoring patient responses in treating urological pathologies, including non-muscle invasive bladder cancer (NMIBC). Yet, analysing urine biomarkers poses several challenges, including ensuring specimen stability during transportation and analytical processing. This prospective feasibility study aimed to investigate how urinary leukocytes and proteins are impacted by storing and refrigerating urine.

Methods

Stability of leukocytes from four healthy donors (HD) spiked into urine supernatants was analyzed for up to 72 h at 4°C. Urine samples from five NMIBC patients undergoing BCG treatment were divided into two portions, followed by either immediate processing or overnight refrigeration. Urinary cell content and soluble factors we

SUBMITTER: Sevko A 

PROVIDER: S-EPMC11662439 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature