Comparative effectiveness of vitrification and slow freezing after heterotopic transplantation of human ovarian tissues.

ABSTRACT:

Background

The aim of this study was to compare the effectiveness of two different vitrification methods and slow freezing in terms of the recovery of endocrine function, follicular morphology and proliferation, apoptosis of stromal cells, and angiogenesis after heterotopic transplantation of human ovarian tissue.

Methods

Ovarian tissue from young women aged 29 to 40 was subjected to two vitrification methods and one slow freezing method. The thawed ovarian tissue was then transplanted into nude mice and divided into three groups (VF1 group, VF2 group, SF group) according to the different freezing methods. Ovarian tissue samples were collected at 4 and 6 weeks post-transplantation. The recovery of ovarian function was evaluated by observing the estrous cycle and measuring es

SUBMITTER: Zeng Y 

PROVIDER: S-EPMC11662470 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature