Profiling of i-motif-binding proteins reveals functional roles of nucleolin in regulation of high-order DNA structures.

ABSTRACT: Non-canonical DNA structures, such as the G-quadruplex (G4) and i-motif (iM), are formed at guanine- and cytosine-rich sequences, respectively, in living cells and involved in regulating various biological processes during the cell cycle. Therefore, the formation and resolution of these non-canonical structures must be dynamically regulated by physiological conditions or factors that can bind G4 and iM structures. Although many G4 binding proteins responsible for tuning the G4 structure have been discovered, the structural regulation of iM by iM-binding proteins remains enigmatic. In this study, we developed a protein-labeling DNA probe bearing an alkyne moiety through a reactive linker, for proximity-labeling of nucleic acid-binding proteins, and searched for new iM-binding proteins. Alky

SUBMITTER: Ban Y 

PROVIDER: S-EPMC11662649 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature