Chemotherapeutic agents and leucine deprivation induce codon-biased aberrant protein production in cancer.

ABSTRACT: Messenger RNA (mRNA) translation is a tightly controlled process frequently deregulated in cancer. Key to this deregulation are transfer RNAs (tRNAs), whose expression, processing and post-transcriptional modifications are often altered in cancer to support cellular transformation. In conditions of limiting levels of amino acids, this deregulated control of protein synthesis leads to aberrant protein production in the form of ribosomal frameshifting or misincorporation of non-cognate amino acids. Here, we studied leucine, an essential amino acid coded by six different codons. Surprisingly, we found that leucine deprivation leads to ribosomal stalling and aberrant protein production in various cancer cell types, predominantly at one codon, UUA. Similar effects were observed after treatment

SUBMITTER: Kochavi A 

PROVIDER: S-EPMC11662694 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature