Human neural stem cells directly programmed from peripheral blood show functional integration into the adult mouse brain.

ABSTRACT: Transplantation of induced pluripotent stem cell-derived neural cells represents a promising strategy for treating neurodegenerative diseases. However, reprogramming of somatic cells and their subsequent neural differentiation is complex and time-consuming, thereby impeding autologous applications. Recently, direct transcription factor-based conversion of blood cells into induced neural stem cells (iNSCs) has emerged as a potential alternative. However, little is known about the functionality of iNSC-derived neurons upon in vivo transplantation. Here, we grafted human iNSCs derived from adult peripheral blood by temporary overexpression of the transcription factors SOX2 and cMYC into the hippocampus or striatum of adult unlesioned immunodeficient Rag2^tm1FwaIl2rg^tm1Wjl

SUBMITTER: Berg LJ 

PROVIDER: S-EPMC11662720 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature