PH-FISH: coupled microscale analysis of microbial identity and acid-base metabolism in complex biofilm samples.

ABSTRACT:

Background

Correlative structural and chemical imaging of biofilms allows for the combined analysis of microbial identity and metabolism at the microscale. Here, we developed pH-FISH, a method that combines pH ratiometry with fluorescence in situ hybridization (FISH) in structurally intact biofilms for the coupled investigation of microbial acid metabolism and biofilm composition. Careful biofilm handling and modified sample preparation procedures for FISH allowed preservation of the three-dimensional biofilm structure throughout all processing and imaging steps. We then employed pH-FISH to investigate the relationship between local biofilm pH and the distribution of acid-producing (streptococci) and acid-consuming (Veillonella spp.) bacteria in dental biofilms from healthy subject

SUBMITTER: Del Rey YC 

PROVIDER: S-EPMC11662800 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature