Dynamic effects of fixed orthodontic treatment on oral health and oral microbiota: a prospective study.

ABSTRACT:

Objective

This prospective clinical study examined the effects of fixed orthodontic appliances on oral hygiene and assessed changes in the oral microflora structure of orthodontic patients using high-throughput sequencing technology.

Methods

A total of 45 patients undergoing initial fixed orthodontic treatment were recruited from three hospitals in Beijing, China. Clinical parameters and oral hygiene habits questionnaire were recorded at pre-treatment (T0) and at a 6-month follow-up (T1). Saliva samples were collected for DNA extraction, PCR amplification, 16S rRNA gene sequencing, and bioinformatics analysis. Microbial diversity and structural changes in abundance were evaluated.

Results

After six months of orthodontic treatment, patients' daily brushing frequency in

SUBMITTER: Zhao M 

PROVIDER: S-EPMC11662850 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature