Transcriptomic heterogeneity of non-beta islet cells is associated with type 2 diabetes development in mouse models.

ABSTRACT:

Aims/hypothesis

The aim of this work was to understand the role of non-beta cells in pancreatic islets at early stages of type 2 diabetes pathogenesis.

Methods

Specific clustering was employed to single-cell transcriptome data from islet cells of obese mouse strains differing in their diabetes susceptibility (diabetes-resistant B6.V.Lep^ob/ob [OB] and diabetes-susceptible New Zealand Obese [NZO] mice) on a diabetogenic diet.

Results

Refined clustering analysis revealed several heterogeneous subpopulations for alpha cells, delta cells and macrophages, of which 133 mapped to human diabetes genes identified by genome-wide association studies. Importantly, a similar non-beta cell heterogeneity was found in a dataset of human islets from donors at different stage

SUBMITTER: Gottmann P 

PROVIDER: S-EPMC11663180 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature