Exosomes derived from hypoxic mesenchymal stem cells restore ovarian function by enhancing angiogenesis.

ABSTRACT:

Background

hucMSC-exosomes can be engineered to strengthen their therapeutic potential, and the present study aimed to explore whether hypoxic preconditioning can enhance the angiogenic potential of hucMSC-exosomes in an experimental model of POF.

Methods

Primary hucMSCs and ROMECs were isolated from fresh tissue samples and assessed through a series of experiments. Exosomes were isolated from hucMSCs under normoxic or hypoxic conditions (norm-Exos and hypo-Exos, respectively) and then characterized using classic experimental methods. Based on a series of angiogenesis-related assays, we found that hypo-Exos significantly promoted ROMEC proliferation, migration, and tube formation and increased angiogenesis-promoting molecules in vitro. Histology, immunohistochemistry, and im

SUBMITTER: Qu Q 

PROVIDER: S-EPMC11663355 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature