MicroRNA expression in extracellular vesicles as a novel blood-based biomarker for Alzheimer's disease.

ABSTRACT:

Introduction

Brain cell-derived small extracellular vesicles (sEVs) in blood offer unique cellular and molecular information related to the onset and progression of Alzheimer's disease (AD). We simultaneously enriched six specific sEV subtypes from the plasma and analyzed a selected panel of microRNAs (miRNAs) in older adults with/without cognitive impairment.

Methods

Total sEVs were isolated from the plasma of participants with normal cognition (CN; n = 11), mild cognitive impairment (MCI; n = 11), MCI conversion to AD dementia (MCI-AD; n = 6), and AD dementia (n = 11). Various brain cell-derived sEVs (from neurons, astrocytes, microglia, oligodendrocytes, pericytes, and endothelial cells) were enriched and analyzed for specific miRNAs.

Results

miRNAs in sEV subtypes

SUBMITTER: Kumar A 

PROVIDER: S-EPMC11663460 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature