A role for root carbonic anhydrase βCA4 in the bicarbonate tolerance of Arabidopsis thaliana.

ABSTRACT: Carbonic anhydrases (CAs) are the main enzymes handling bicarbonate in the different cell compartments. This study analyses the expression of CAs in roots of Arabidopsis thaliana demes differing in tolerance to bicarbonate: the tolerant A1_(C+) deme and the sensitive deme, T6_(C-). Exposure to 10 mM NaCl caused a transient depolarization of the root cell membranes, and in contrast, the supply of 10 mM NaHCO₃ caused hyperpolarization. This hyperpolarization was much stronger in A1_(C+) than in T6_(C-). Acetazolamide (AZ), a specific inhibitor of CAs, abolished the hyperpolarizing effect in A1_(C+), indicating the implication of CAs in this fast membrane response. The time-dependent (3 to 72 h) expression profiles of 14 CAs in root

SUBMITTER: Perez-Martin L 

PROVIDER: S-EPMC11663626 | biostudies-literature | 2024 Nov-Dec

REPOSITORIES: biostudies-literature