Fractionated alpha and mixed beam radiation promote stronger pro-inflammatory effects compared to acute exposure and trigger phagocytosis.

ABSTRACT:

Introduction and methods

Aiming to evaluate safety aspects of a recently proposed approach to target Alzheimer's disease, we mimicked a complex boron neutron capture therapy field using a mixed beam consisting of high- and low-linear energy transfer (LET) radiation, ²⁴¹Am alpha particles (α) and/or X-ray radiation respectively, in human microglial (HMC3) cells.

Results

Acute exposure to 2 Gy X-rays induced the strongest response in the formation of γH2AX foci 30 min post irradiation, while α- and mixed beam-induced damage (α:X-ray = 3:1) sustained longer. Fractionation of the same total dose (0.4 Gy daily) induced a similar number of γH2AX foci as after acute radiation, however, α- or mixed irradiation caused a higher expression of DNA damage response genes CDKN1A

SUBMITTER: Karimi Roshan M 

PROVIDER: S-EPMC11663654 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature