Patterns of the within-host evolution of human norovirus in immunocompromised individuals and implications for treatment.

ABSTRACT:

Background

Currently, there is no licensed treatment for chronic norovirus infections, but the use of intra-duodenally-delivered immunoglobulins is promising; nevertheless, varying results have limited their wide use. Little is known about the relationship between norovirus genetic diversity and treatment efficacy.

Methods

We analyzed the norovirus within-host diversity and evolution in a cohort of 20 immunocompromised individuals using next-generation sequencing (NGS) and clone-based sequencing of the capsid (VP1) gene. Representative VP1s were expressed and their glycan receptor binding affinity and antigenicity were evaluated.

Findings

The P2 domain, within the VP1, accumulated up to 30-fold more non-synonymous mutations than other genomic regions. Intra-host virus

SUBMITTER: Izquierdo-Lara RW 

PROVIDER: S-EPMC11663770 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature