Effect of plasma-induced oxidation on NK cell immune checkpoint ligands: A computational-experimental approach.

ABSTRACT: Non-thermal plasma (NTP) shows promise as a potent anti-cancer therapy with both cytotoxic and immunomodulatory effects. In this study, we investigate the chemical and biological effects of NTP-induced oxidation on several key, determinant immune checkpoints of natural killer (NK) cell function. We used molecular dynamics (MD) and umbrella sampling simulations to investigate the effect of NTP-induced oxidative changes on the MHC-I complexes HLA-Cw4 and HLA-E. Our simulations indicate that these chemical alterations do not significantly affect the binding affinity of these markers to their corresponding NK cell receptor, which is supported with experimental read-outs of ligand expression on human head and neck squamous cell carcinoma cells after NTP application. Broadening our scope to othe

SUBMITTER: Heirman P 

PROVIDER: S-EPMC11663777 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature