In vitro modelling of anterior primitive streak patterning with human pluripotent stem cells identifies the path to notochord progenitors.

ABSTRACT: Notochord progenitors (NotoPs) represent a scarce yet crucial embryonic cell population, playing important roles in embryo patterning and eventually giving rise to the cells that form and maintain intervertebral discs. The mechanisms regulating NotoPs emergence are unclear. This knowledge gap persists due to the inherent complexity of cell fate patterning during gastrulation, particularly within the anterior primitive streak (APS), where NotoPs first arise alongside neuro-mesoderm and endoderm. To gain insights into this process, we use micropatterning together with FGF and the WNT pathway activator CHIR9901 to guide the development of human embryonic stem cells into reproducible patterns of APS cell fates. We show that CHIR9901 dosage dictates the downstream dynamics of endogenous TGFβ si

SUBMITTER: Robles-Garcia M 

PROVIDER: S-EPMC11664173 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature