Chemogenetic activation of astrocytes modulates sleep-wakefulness states in a brain region-dependent manner.

ABSTRACT:

Study objectives

Astrocytes change their intracellular calcium (Ca²⁺) concentration during sleep/wakefulness states in mice. Furthermore, the Ca²⁺ dynamics in astrocytes vary depending on the brain region. However, it remains unclear whether alterations in astrocyte activity can affect sleep-wake states and cortical oscillations in a brain region-dependent manner.

Methods

Astrocyte activity was artificially manipulated in mice using chemogenetics. Astrocytes in the hippocampus and pons, which are 2 brain regions previously classified into different clusters based on their Ca²⁺ dynamics during sleep-wakefulness, were focused on to compare whether there are differences in the effects of astrocytes from different brain regions.

Results

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SUBMITTER: Kurogi Y 

PROVIDER: S-EPMC11664484 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature