Optimization of the large-scale production for Erwinia amylovora bacteriophages.

ABSTRACT:

Background

Fire blight, caused by Erwinia amylovora, poses a significant threat to global agriculture, with antibiotic-resistant strains necessitating alternative solutions such as phage therapy. Scaling phage therapy to an industrial level requires efficient mass-production methods, particularly in optimizing the seed culture process. In this study, we investigated large-scale E. amylovora phage production by optimizing media supplementation and fermenter conditions, focusing on minimizing seed phages and pathogenic strains to reduce risks and improve the seed culture process.

Results

We optimized the phage inoculum concentrations and media supplements to achieve higher phage yields comparable to or exceeding conventional methods. Laboratory-scale validation and refinement

SUBMITTER: Jo SJ 

PROVIDER: S-EPMC11664846 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature