H₂-driven xylitol production in Cupriavidus necator H16.

ABSTRACT:

Background

Biocatalysis offers a potentially greener alternative to chemical processes. For biocatalytic systems requiring cofactor recycling, hydrogen emerges as an attractive reducing agent. Hydrogen is attractive because all the electrons can be fully transferred to the product, and it can be efficiently produced from water using renewable electricity. In this article, resting cells of Cupriavidus necator H16 harboring a NAD-dependent hydrogenase were employed for cofactor recycling to reduce D-xylose to xylitol, a commonly used sweetener. To enable this bioconversion, D-xylose reductase from Scheffersomyces stipitis was heterologously expressed in C. necator.

Results

D-xylose reductase was successfully expressed in C. necator, enabling almost complete bioconversion of 30

SUBMITTER: Jamsa T 

PROVIDER: S-EPMC11665087 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature