Establishment and application of a one-step multiplex real-time PCR assay for detection of A, B, and C subtypes of avian metapneumovirus.

ABSTRACT: Avian metapneumovirus (aMPV) represents a significant threat to the poultry industry, exhibiting a high degree of genetic diversity. Of these, the aMPV types A (aMPV-A), B (aMPV-B) and C (aMPV-C) are frequently detected in Chinese waterfowl and live poultry markets. Therefore, the rapid and accurate identification of these subtypes is of paramount importance in order to halt the spread of the disease. In this study, we have developed a multiplex real-time PCR assay endowed with the capacity to simultaneously discriminate aMPV-A, aMPV-B, and aMPV-C. This method demonstrates remarkable specificity, selectively amplifying aMPV-A, aMPV-B, and aMPV-C without cross-reactivity with other common avian pathogens. Furthermore, this method exhibits high sensitivity, with a detection threshold of 8.5

SUBMITTER: Su M 

PROVIDER: S-EPMC11665345 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature