Impact of electro-acupuncture on EAAT2 and NMDAR-2B expression in goats with visceral hypersensitivity.

ABSTRACT:

Objective

This study evaluates the effect of electro-acupuncture (EA) on visceral hypersensitivity (VH) and the expression of N-methyl-D-aspartate receptor-2B (NMDAR-2B) and glutamate transporter EAAT2 in goats.

Methods

Twenty-four goats were divided into four groups: saline, 2, 4, 6-Trinitrobenzenesulfonic acid (TNBS), TNBS + EA, and sham EA. EA was administered at Zusanli (ST36) with 60 Hz and 1-3 mA on specified days. Electromyography (EMG) recorded visceromotor response to colorectal distention (CRD). Spinal cords were collected for immunohistochemistry, western blotting, and RT-PCR. The ileum was examined histologically.

Results

The repeated EA administration significantly attenuated VH (P < 0.05) in TNBS-treated goats without similar effects in the sham group. N

SUBMITTER: Tahir AH 

PROVIDER: S-EPMC11665384 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature