Targeted and Untargeted Amine Metabolite Quantitation in Single Cells with Isobaric Multiplexing.

ABSTRACT: We developed a single cell amine analysis approach utilizing isobarically multiplexed samples of 6 individual cells along with analyte abundant carrier. This methodology was applied for absolute quantitation of amino acids and untargeted relative quantitation of amines in a total of 108 individual cells using nanoflow LC with high-resolution mass spectrometry. Together with individually determined cell sizes, this provides accessible quantification of intracellular amino acid concentrations within individual cells. The targeted method was partially validated for 10 amino acids with limits of detection in low attomoles, linear calibration range covering analyte amounts typically from 30 amol to 120 fmol, and correlation coefficients (R) above 0.99. This was applied with cell sizes recorded

SUBMITTER: Heininen J 

PROVIDER: S-EPMC11665497 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature