A generally applicable validation scheme for the assessment of factors involved in reproducibility and quality of DNA-microarray data.

ABSTRACT:

Background

In research laboratories using DNA-microarrays, usually a number of researchers perform experiments, each generating possible sources of error. There is a need for a quick and robust method to assess data quality and sources of errors in DNA-microarray experiments. To this end, a novel and cost-effective validation scheme was devised, implemented, and employed.

Results

A number of validation experiments were performed on Lactococcus lactis IL1403 amplicon-based DNA-microarrays. Using the validation scheme and ANOVA, the factors contributing to the variance in normalized DNA-microarray data were estimated. Day-to-day as well as experimenter-dependent variances were shown to contribute strongly to the variance, while dye and culturing had a relatively modest contrib

SUBMITTER: van Hijum SA 

PROVIDER: S-EPMC1166551 | biostudies-literature | 2005 May

REPOSITORIES: biostudies-literature