Gpnmb and Spp1 mark a conserved macrophage injury response masking fibrosis-specific programming in the lung.

ABSTRACT: Macrophages are required for healthy repair of the lungs following injury, but they are also implicated in driving dysregulated repair with fibrosis. How these 2 distinct outcomes of lung injury are mediated by different macrophage subsets is unknown. To assess this, single-cell RNA-Seq was performed on lung macrophages isolated from mice treated with LPS or bleomycin. Macrophages were categorized based on anatomic location (airspace versus interstitium), developmental origin (embryonic versus recruited monocyte derived), time after inflammatory challenge, and injury model. Analysis of the integrated dataset revealed that macrophage subset clustering was driven by macrophage origin and tissue compartment rather than injury model. Gpnmb-expressing recruited macrophages that were enriched fo

SUBMITTER: King EM 

PROVIDER: S-EPMC11665561 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature