A computer program for the estimation of protein and nucleic acid sequence diversity in random point mutagenesis libraries.

ABSTRACT: A computer program for the generation and analysis of in silico random point mutagenesis libraries is described. The program operates by mutagenizing an input nucleic acid sequence according to mutation parameters specified by the user for each sequence position and type of point mutation. The program can mimic almost any type of random mutagenesis library, including those produced via error-prone PCR (ep-PCR), mutator Escherichia coli strains, chemical mutagenesis, and doped or random oligonucleotide synthesis. The program analyzes the generated nucleic acid sequences and/or the associated protein library to produce several estimates of library diversity (number of unique sequences, point mutations, and single point mutants) and the rate of saturation of these diversities during experimen

SUBMITTER: Volles MJ 

PROVIDER: S-EPMC1166583 | biostudies-literature | 2005

REPOSITORIES: biostudies-literature