Kinetic principles of chemical cross-link formation for protein-protein interactions.

ABSTRACT: Proteins play a central role in most biological processes within the cell, and deciphering how they interact is key to understand their function. Cross-linking coupled with mass spectrometry is an essential tool for elucidating protein-protein interactions (PPIs). Despite its importance, we still know surprisingly little about the principles that underlie the process of chemical cross-link formation itself and how it is influenced by different physicochemical factors. To understand the molecular details of cross-link formation, we have set up a comprehensive kinetic model and carried out simulations of protein cross-linking on large protein complexes. We dissect the contribution on the cross-link yield of parameters such as amino acid reactivity, cross-linker concentration, and hydrolysis

SUBMITTER: Kammer KM 

PROVIDER: S-EPMC11665911 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature