Basement membranes are crucial for proper olfactory placode shape, position and boundary with the brain, and for olfactory axon development.

ABSTRACT: Despite recent progress, the complex roles played by the extracellular matrix in development and disease are still far from being fully understood. Here, we took advantage of the zebrafish sly mutation which affects Laminin γ1, a major component of basement membranes, to explore its role in the development of the olfactory system. Following a detailed characterisation of Laminin distribution in the developing olfactory circuit, we analysed basement membrane integrity, olfactory placode and brain morphogenesis, and olfactory axon development in sly mutants, using a combination of immunochemistry, electron microscopy and quantitative live imaging of cell movements and axon behaviours. Our results point to an original and dual contribution of Laminin γ1-dependent basement membra

SUBMITTER: Tignard P 

PROVIDER: S-EPMC11666240 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature