Induction of cardiac alternans in human iPS-derived cardiomyocytes through β-adrenergic receptor stimulation.

ABSTRACT: Cardiac alternans (C-ALT) is a phenomenon of alternating strong and weak contractions in the heart and is considered a risk factor for the development of heart failure and arrhythmias. However, no model has been reported that can induce C-ALT in vitro using human cells, and the developmental mechanism of C-ALT has not been studied using human cells. In this study, we successfully induced C-ALT in vitro using human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). By stimulating β-adrenergic receptor with isoproterenol on hiPSC-CMs cultured in atmospheric condition (with ~0.04% CO₂), contractility and calcium transient were observed to alternately increase and decrease with each beat. In contrast, C-ALT was not induced in hiPSC-CMs cultured at 5% CO₂ co

SUBMITTER: Hinata Y 

PROVIDER: S-EPMC11666346 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature