Enhancing the reverse transcriptase function in Taq polymerase via AI-driven multiparametric rational design.

ABSTRACT:

Introduction

Modification of natural enzymes to introduce new properties and enhance existing ones is a central challenge in bioengineering. This study is focused on the development of Taq polymerase mutants that show enhanced reverse transcriptase (RTase) activity while retaining other desirable properties such as fidelity, 5'- 3' exonuclease activity, effective deoxyuracyl incorporation, and tolerance to locked nucleic acid (LNA)-containing substrates. Our objective was to use AI-driven rational design combined with multiparametric wet-lab analysis to identify and validate Taq polymerase mutants with an optimal combination of these properties.

Methods

The experimental procedure was conducted in several stages: 1) On the basis of a foundational paper, we selected 18 candida

SUBMITTER: Tomilova YE 

PROVIDER: S-EPMC11666352 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature