Efficient gene deletion of Integrin alpha 4 in primary mouse CD4 T cells using CRISPR RNA pair-mediated fragmentation.

ABSTRACT: The functional specialization of CD4 T lymphocytes into various subtypes, including T_H1 and T_FH cells, is crucial for effective immune responses. T_FH cells facilitate B cell differentiation within germinal centers, while T_H1 cells are vital for cell-mediated immunity against intracellular pathogens. Integrin α4, a cell surface adhesion molecule, plays significant roles in cell migration and co-stimulatory signaling. In this study, we investigated the role of Integrin α4 in regulating T_FH and T_H1 cell populations during acute viral infection using CRISPR-Cas9 gene editing. To effectively delete the Itga4 in primary mouse CD4 T cells, we selected various combinations of crRNAs and generated ribonucleoprotein complexes w

SUBMITTER: Wi T 

PROVIDER: S-EPMC11666438 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature