Decoding the prognostic landscape of LUAD: the interplay between N⁶-methyladenosine modification and immune microenvironment.

ABSTRACT:

Background

To determine the role of N⁶-methyladenosine (m⁶A) modification in the tumor immune microenvironment (TIME), as well as their association with lung adenocarcinoma (LUAD).

Methods

Consensus clustering was performed to identify the subgroups with distinct immune or m⁶A modification patterns using profiles from TCGA. A risk score model was constructed using least absolute shrinkage and selection operator regression and validated in two independent cohorts and LUAD tissue microarrays. For experimental validation, the regulation of METTL3/m⁶A axis in the expression of candidate genes by RIP-qPCR assay in A549 and H460 cell lines. Co-culture experiments with human T cells were performed to evaluate the impact of METTL3 on the e

SUBMITTER: Chen Q 

PROVIDER: S-EPMC11666524 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature